Akin, Ali TuğrulKaymak, EminKarabulut, DeryaDoğanyiğit, ZüleyhaCeylan, TayfunToluk, AyşeÖzdamar, Saim2021-03-102021-03-102020Ali, A. K. I. N., Kaymak, E., Karabulut, D., Doğanyiğit, Z., Ceylan, T., Toluk, A., & Özdamar, S. Comparison of the acute and cumulative dose administrations in doxorubicin-induced hepatotoxicity via evaluation of the histopathological changes and inflammation in rats. Experimental and Applied Medical Science, 1(3), 73-81.https://doi.org/10.46871/eams.2020.10https://hdl.handle.net/20.500.12695/991Doxorubicin (DOX) may lead to hepatotoxicity when administered chronically or in a high dose. The aim of this study is to determine dose–dependent effects of DOX in rat liver tissue. Thirty male Wistar albino rats were divided into three groups; group I as control, group II as receiving chronically DOX (2 mg/kg, twice in a week, total 20 mg/kg, intraperitoneally) and group III as receiving an acute–single dose of DOX (15 mg/kg, intraperitoneally, on the 20th day) administered groups. At the end of 30th day, animals were sacrificed, and liver tissues were extracted for histopathological and immunohistochemical evaluation. Sections were stained with hematoxylin & eosin to evaluate the histopathological changes and TNF-? and IL-6 expressions were detected by immunohistochemical staining. Both chronic and acute administrations of DOX triggered a significant liver damage. However, it was observed that liver damage induced by acute–single dose DOX administrations were higher than those induced by chronic DOX administrations. TNF-? and IL-6 immunoreactivity was significantly increased in both group II and III group compared to control group. However, immunoreactivity of TNF-? was substantially higher in the group III compared to control. These results demonstrated that acute administrations of DOX relatively induce serious liver damage and inflammatory response.eninfo:eu-repo/semantics/openAccessDoxorubicinHepatotoxicityInflammationLiver damageArticle137381