Ceylan, TayfunÜnsal, MuratKaymak, EminAkin, Ali TuğrulKuloğlu, NurhanKarabulut, DeryaYakan, Birkan2025-01-072025-01-072024CEYLAN, T., ÜNSAL, M., KAYMAK, E., AKİN, A. T., KULOĞLU, N., KARABULUT, D., & YAKAN, B. (2024). The Research on Endoplasmic Reticulum Stress-mediated Protective Effect of Melatonin against Cardiotoxicity Following Cisplatin Treatment. Medical Journal of Bakirkoy, 20(4), 333–339.https://doi.org/10.4274/BMJ.galenos.2024.2024.5-1https://hdl.handle.net/20.500.12695/3129Objective: Cisplatin (CP) is a chemotherapeutic drug that causes cardiotoxicity. Melatonin (MEL) is secreted by the pineal gland throughout the night. This study aimed to investigate the protective effect of MEL against cardiotoxicity associated with CP exposure. Methods: Physiological saline was applied to Group 1 (control) throughout the experiment. A single dose of CP (7 mg/kg) was administered to Group 2 on the 5th day of the experiment. Group 3 received MEL (10 mg/kg) for 7 days and CP (7 mg/kg) on day 5. MEL (10 mg/kg) was administered to group 4 for 7 days. On the 8th day of the experiment, the hearts were removed under anesthesia. Sections taken from heart tissue samples were stained with hematoxylin and eosin for histopathological evaluation. Additionally, heart tissue sections were immunohistochemically stained for 78-kDa glucose-regulated protein (GRP-78), growth arrest and DNA damage-inducible gene 153 (GADD 153), and connexins (Cx 43) expression. Results: CP application caused cellular damage and disrupted heart tissue tissue integrity. At the same time, CP application caused an increase in the expression of GRP-78 and GADD 153, whereas it caused a decrease in the expression of Cx43. MEL application heals cell damage and impaired tissue integrity. However, while reducing GRP-78 and GADD153 expression; CX had an effect of increasing expression. Conclusion: MEL may have a protective effect against CP-induced cardiotoxicity in rats.eninfo:eu-repo/semantics/openAccessCardiotoxicityCisplatinEndoplasmic Reticulum StressMelatoninRatArticle204333339