Researches on the protective effect of caffeic acid phenethyl ester on testicular 2 damage caused by cisplatin

Abstract

BACKGROUND/AIM:Cisplatin (CP), a chemotherapeutic drug, causes damage to spermatogenic serial cells, Sertoli cells and Leydig cells in rat testicles. We aimed to investigate the protective effect of caffeic acid phenethyl ester (CAPE), one of the active ingredients of propolis, in eliminating CP-induced testicular damage. MATERIALS AND METHODS:Group 1 (control group) was given Serum Physiological intraperitoneal (ip) throughout the experiment. Group 2 (CP group) was given a single dose of CP (7 mg / kg) (ip) on the 7th day. Group 3 (CP+CAPE group), CAPE (10 µmol / kg / day) (ip) for 12 days and single dose CP (7 mg / kg) ip on day 7. given as. Group 4 (CAPE group) was given CAPE (10 µmol / kg / day) (ip) for 12 days. On the 14th day of the experiment, the rats were decapitated under xylazine and ketamine anesthesia and their testicles were removed. The sections obtained from the testicles were stained with hematoxylin-eosin and histopathological damage was evaluated. Malondialdehyde (MDA) levels and superoxide dismutase (SOD), catalase (CAT) enzymatic activities were measured in testicular tissue samples. Testosterone (TES) levels were measured in blood serum. Johnsen testicular biopsy score (JTBS) was used to evaluate testicular tubules. DNA damage was evaluated in sperm samples taken from the ductus epididymis by comet assay technique. RESULTS:In Group 2 given CP, the testicles were severely damaged. It was observed that histological damage was reduced in testes by giving CAPE in Group 3. In addition, according to JTBS, the value was significantly higher in testicular tubules (p < 0.05). Moreover, the MDA level was decreased in the Group 3. But, SOD, CAT and TES levels were increased in the Group 3. DNA damage also decreased significantly in Group 3 compared to Group 2 (p < 0.05). CONCLUSION:As a result, CAPE may be protective against damage caused by CP in the testicles.