Evaluation Of Epididymitis Tissue Of Cisplatin-Induced Rats
Citation
T. CEYLAN, D. KARABULUT, E. ÖZTÜRK, A. T. AKİN, E. KAYMAK, and B. YAKAN, “Evaluation of Epididymitis Tissue of Cisplatin Induced Rats,” presented at the 2nd International Congress on Sports, Anthropology, Nutrition, Anatomy and Radiology (SANAR2020)., NEVŞEHİR, 2020.Abstract
As a chemotherapeutic agent, cisplatin is an antineoplastic agent used in the treatment of
various solid tumors such as lung, testicle and bladder cancer (Ezaki, Nishiumi, Azuma, &
Yoshida, 2017; Salem, Helmi, & Assaf, 2018). Autophagy abnormality is one of the
mechanisms of cell death leading to the development of diseases such as cancer (Yang &
Klionsky, 2010). In this study, it was aimed to determine the damage caused by cisplatin on
tissue by using autophagy inhibitor and activator.
A total of 24 Wistar albino male rats were used in the study, 6 animals in each group. Group I;
Control, Group II; Cisplatin (8 mg / kg), Group III; Rapamycin (2 mg / kg), Group IV; Group
of 3-methylated (15 mg / kg). While rapamycin and 3-methyladenine were administered for 15
days, cisplatin was applied to these groups on the 7th day of a single experiment. At the end of
the experiment, the organs of the rats, which were anesthetized, were removed and placed in
formaldehyde for histological follow-up. Hematoxylin-Eosin and Heat-shock protein70
(HSP70) immunohistochemistry was applied to the sections taken after histological techniques.
Group I tissue sections had a normal histological appearance. In Group II sections, some areas
were disintegrated in the tubular basement membrane and vacuolization was observed in the
tubule. In addition, some epithelial cells were observed to be more eosinophilic. Tissue sections
of groups III and IV had a more regular appearance as epithelialization, tubule basement
membrane. Eosinophilic cells were observed in tubular epithelialization of group IV. HSP70
immunoreactivity was observed in the inter-tubular connective tissue of all groups. HSP70
immunoreactivity was observed in the tubular epithelium of both Group III and Group IV tissue
sections, mostly in Group II.
Sperm transport, maturation and storage is the most important task of the epididymis (Wang &
Kumar, 2012). It was concluded that the organ was affected by agents such as cisplatin to
maintain semen quality and potentiality of spermatozoa. We think that HSP70 mediates the
formation of autophagy that occurs in the organ.