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    Isolation and characterization a novel Acinetobacter bacteriophage with activity against several multidrug-resistant Gram-negative bacteria
    (Yelda İşlek, 06/2025) Hamzeli, Nur; Aktaş, Ahmet; Köksal Çakırlar, Fatma
    Acinetobacter baumannii (A. baumannii) is a notorious nosocomial pathogen that is frequently associated with multidrug resistance around the world. Endolysin is a bacteriophage-produced hydrolytic enzyme. In this study, an urban waste water sample (1 L) was collected from Istanbul. A double-agar plating technique was used for host range analysis. The purified genomic DNA sequences were performed on the Miseq. De novo assembly of the crude sequences yielded a double-stranded DNA molecule with a length of 45,679 bp and a guanine-cytosine content of 37.6%. Genome annotation revealed that the vB_AbaM_YNAF genome comprises 85 open reading frames, 23 of which are functional and do not define any tRNA-rRNA genes. Electron microscopy examination and phylogenetic analysis of the genome revealed that vB_AbaM_YNAF represents a novel origin of an unclassified Obolenskvirus belonging to the class Caudoviricetes. vB_AbaM_YNAF infected one reference and three different multidrug-resistant (MDR) strains of each of A. baumannii and K. pneumoniae. However, it did not have any effect on a reference E. coli strain. Based on all of these findings, LysYAN could be a potential agent for treating MDR-Gram-negative bacteria. Further investigations on vB_AbaM_YNAF may be beneficial for designing an alternative weapon with probable wide host-range activity to fight MDR infections.