Mitigative effects of chloroquine treatment against hypoxia-induced intestinal injury: a histological and immunohistochemical study

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dc.authorid0000-0002-0917-0378
dc.contributor.authorAkin, Ali Tuğrul
dc.contributor.authorKaymak, Emin
dc.contributor.authorÖztürk, Emel
dc.contributor.authorCeylan, Tayfun
dc.contributor.authorYalçın, Betül
dc.contributor.authorBaşaran, Kemal Erdem
dc.contributor.authorKarabulut, Derya
dc.contributor.authorDoğanyiğit, Züleyha
dc.contributor.authorÖzdamar, Saim
dc.contributor.authorYakan, Birkan
dc.date.accessioned2022-03-31T20:54:28Z
dc.date.available2022-03-31T20:54:28Z
dc.date.issued2022en_US
dc.departmentKapadokya Üniversitesi, Kapadokya Meslek Yüksekokulu, Patoloji Laboratuvar Teknikleri Bölümü
dc.description.abstractObjective: Hypoxia has an important role in the disruption of intestinal mucosal integrity because of inflammation and apoptosis induced by inflammatory cytokines such as TNF-? (Tumor necrosis factor-alpha), IL-6 and IFN-y, and apoptotic regulatory proteins. Chloroquine (CLQ) is a drug used in the novel coronavirus disease (COVID-19) and is widely used for the treatment of many inflammatory diseases such as malaria and rheumatoid arthritis. In this study, we aimed to reduce the destructive effects of hypoxia-induced inflammation and apoptosis in the intestinal mucosa of rats with CLQ applications. Methods: For this purpose, a total of 24 Wistar Albino rats were randomly divided into three groups; Group I: Control group (n=8), Group II: Hypoxia (n=8) and Group III: Hypoxia + CLQ (n=8). The control group was housed in plexiglass cages to keep the oxygen levels at 10% levels for 28 days, while the hypoxia and hypoxia+CLQ groups were housed in a normal atmospheric environment (21% O2), and the hypoxia+CLQ group was administered CLQ at a dose of 50 mg/kg every day for 28 days. At the end of the experiment, the intestinal tissues of the experimental animals, were extracted under the anesthesia and they were sacrificed. Results: As a result of histopathological evaluations, it was determined that CLQ applications showed healing properties on the histopathological effects induced by hypoxia in the intestine. While an increase in TNF-? expression was observed in the hypoxia group, a statistically significant decrease was detected in the hypoxia+CLQ group. In addition, Bax expression was found to be statistically significantly lower in the hypoxia+CLQ group when compared to the hypoxia group. On the contrary, it was observed that Bcl-2 expression was statistically significantly increased in the hypoxia+CLQ group compared to the Hypoxia group. Conclusion: We observed that hypoxia causes significant damage to the intestinal mucosa and triggers a severe inflammation that drives cells to apoptosis. Considering the curative effects of chloroquine on the intestinal mucosa, we suggest that this anti-inflammatory drug has a potential to use clinically to alleviate the deleterious effects of hypoxia in the intestine.
dc.identifier.citationAKİN, A. T., KAYMAK, E., OZTURK, E., CEYLAN, T., YALCIN, B., BASARAN, K. E., ... & YAKAN, B. Mitigative effects of chloroquine treatment against hypoxia-induced intestinal injury: A histological and immunohistochemical study. Turkish Bulletin of Hygiene and Experimental Biology, 79(1), 59-70.
dc.identifier.endpage70en_US
dc.identifier.issue1en_US
dc.identifier.startpage59en_US
dc.identifier.urihttps://doi.org/10.5505/TurkHijyen.2022.78476
dc.identifier.urihttps://hdl.handle.net/20.500.12695/1563
dc.identifier.volume79en_US
dc.institutionauthorCeylan, Tayfun
dc.language.isoen
dc.publisherTürk Hijyen ve Deneysel Biyoloji Dergisi
dc.relation.ispartofTürk Hijyen ve Deneysel Biyoloji Dergisi
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectApoptosis
dc.subjectCloroquine
dc.subjectHypoxia
dc.subjectTNF-?
dc.subjectIntestine
dc.titleMitigative effects of chloroquine treatment against hypoxia-induced intestinal injury: a histological and immunohistochemical study
dc.title.alternativeHipoksiye bağlı bağırsak hasarına karşı klorokin tedavisinin hafifletici etkileri: histolojik ve immünohistokimyasal bir çalışma
dc.typeArticle

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