A Preliminary Study of A Lactic Acid Biosensor for the Early Detection of Dental Caries

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dc.contributor.authorKÖKBAŞ, Umut
dc.date.accessioned2023-11-09T07:12:56Z
dc.date.available2023-11-09T07:12:56Z
dc.date.issued2023en_US
dc.departmentKapadokya Üniversitesi
dc.description.abstractStatement of Problem: Many mechanisms in the formation of dental caries, but the common point of these mechanisms is the proliferation of microorganisms in the mouth and mostly on the tooth surface. Objective: Although there are approximately 200 to 300 different microorganisms in human dental plaque, Neisseria spp. and S. mutans. The common point of these microorganisms is that they produce lactic acid. In this study, it is aimed to use a biosensor, which is a bioelectrochemical measurement system that combines the measurement capability of physical analysis systems and the specificity of biochemical systems. Material-Method: In this study, lactate oxidase enzyme was immobilised on the gold working electrode surface by means of bovine serum albumin and gelatine. The enzyme was arrested on the electrode surface with the aid of the cross-linking agent glutaraldehyde. Cyclic voltammetry current scanning was performed in lactic acid biosensor preliminary studies. Optimisation studies were conducted in the current range obtained from the peak in the cyclic voltammogram. Within the scope of the optimisation studies, optimum operating temperature and optimum operating pH of the biosensor were tested. Results: An anodic peak at +0.2 V potential was obtained in the cyclic voltammogram drawn according to the reaction on the working electrode surface. In the optimisation studies conducted, it was found that the optimum operating conditions of the biosensor were at 35 °C and pH 6.5. Conclusion: According to the data obtained from the pre-study of the bioelectrochemical method, the concentration of lactic acid in the solution can be determined. With the completion of further studies, a portable lactic acid biosensor can be made. As a result, as a result of the preliminary study, the lactic acid biosensors designed to determine in vitro lactic acid for the early diagnosis of dental caries in the future is promising.
dc.identifier.citationKökbaş, U. (2023). A Preliminary Study of A Lactic Acid Biosensor for the Early Detection of Dental Caries. Cappadocia Health Science Journal, 1(2), 55-63.
dc.identifier.endpage63en_US
dc.identifier.issue2en_US
dc.identifier.startpage55en_US
dc.identifier.urihttps://doi.org/10.58241/ksbd.6
dc.identifier.urihttps://hdl.handle.net/20.500.12695/2339
dc.identifier.volume1en_US
dc.language.isoen
dc.publisherKapadokya Üniversitesi
dc.relation.ispartofKapadokya Sağlık Bilimleri Dergisi
dc.relation.publicationcategoryMakale - Uluslararası - Editör Denetimli Dergi
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.rights.urihttps://creativecommons.org/licenses/by-nc-sa/4.0/*
dc.subjectBiosensor
dc.subjectDental Caries
dc.subjectLactic Acid
dc.subjectLactate Oxidase
dc.titleA Preliminary Study of A Lactic Acid Biosensor for the Early Detection of Dental Caries
dc.typeArticle

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