Chloroquine attenuates chronic hypoxia-induced testicular damage via suppressing endoplasmic reticulum stress and apoptosis in experimental rat model

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dc.authorid0000-0002-0917-0378
dc.contributor.authorAkin, Ali Tuğrul
dc.contributor.authorKaymak, Emin
dc.contributor.authorCeylan, Tayfun
dc.contributor.authorÖztürk, Emel
dc.contributor.authorBaşaran, Kemal Erdem
dc.contributor.authorKarabulut, Derya
dc.contributor.authorÖzdamar, Saim
dc.contributor.authorYakan, Birkan
dc.date.accessioned2022-06-14T14:18:56Z
dc.date.available2022-06-14T14:18:56Z
dc.date.issued2022en_US
dc.departmentKapadokya Üniversitesi, Kapadokya Meslek Yüksekokulu, Patoloji Laboratuvar Teknikleri Bölümü
dc.description.abstractChronic hypoxia negatively affects male fertility by causing pathological changes in male reproductive system. However, underlying mechanisms of this damage are unknown. Chloroquine (CLQ) is an anti-inflammatory agent that is widely used in the treatment of inflammation-related diseases such as malaria and rheumatoid arthritis. This study aimed to investigate the therapeutic effects of CLQ in the hypoxiainduced testicular damage via assessment of hypoxic response, endoplasmic reticulum stress and apoptosis. For this purpose, 32 Wistar albino rats were divided into 4 groups as control (given 20%-21% O2, no treatment), CLQ (given 50 mg/kg and 20%-21% O2 for 28 days), hypoxia (HX) (given 10% O2 for 28 days) and HX + CLQ (given 50 mg/kg and 10% O2 for 28 days). After the experiment, blood samples and testicular tissues were taken. Histopathological evaluation was performed on testicular tissues and hypoxia-inducible factor 1-? (HIF1-?), heat shock proteins (HSPs) HSP70, HSP90 and growth arrest and DNA damage-inducible gene 153 (GADD153) expression levels were detected via immunohistochemistry. Moreover, apoptotic cells were detected via terminal deoxynucleotidyl transferase dUTP nick end labelling (TUNEL) staining and serum testosterone levels were determined by enzyme-linked immunosorbent assay (ELISA) assay. Histopathological changes, apoptotic cell numbers and HIF1-?, HSP70, HSP90 and GADD153 expressions significantly increased in HX group (P < .05). Moreover, serum testosterone levels decreased in this group (P > .05). However, CLQ exerted a strong ameliorative effect on all parameters in HX + CLQ group. According to our results, we suggested that CLQ can be considered as an alternative protective agent for eliminating the negative effects of hypoxic conditions on male fertility.
dc.identifier.citationAkin, A. T., Kaymak, E., Ceylan, T., Ozturk, E., Basaran, K. E., Karabulut, D., ... & Yakan, B. (2022). Chloroquine attenuates chronic hypoxia?induced testicular damage via suppressing endoplasmic reticulum stress and apoptosis in experimental rat model. Clinical and Experimental Pharmacology and Physiology.
dc.identifier.doi10.1111/1440-1681.13669
dc.identifier.endpage11en_US
dc.identifier.issue1en_US
dc.identifier.startpage1en_US
dc.identifier.urihttps://doi.org/10.1111/1440-1681.13669
dc.identifier.urihttps://hdl.handle.net/20.500.12695/1621
dc.identifier.volume11en_US
dc.identifier.wosWOS:000807828500001
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Sceince
dc.institutionauthorCeylan, Tayfun
dc.language.isoen
dc.publisherWiley Online Library
dc.relation.ispartofClinical and Experimental Pharmacology and Physiology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectApoptosis
dc.subjectChloroquine
dc.subjectHeat-shock proteins
dc.subjectHypoxia
dc.subjectMale infertility
dc.titleChloroquine attenuates chronic hypoxia-induced testicular damage via suppressing endoplasmic reticulum stress and apoptosis in experimental rat model
dc.typeArticle

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