Designing siRNA-conjugated plant oil-based nanoparticles for gene silencing and cancer therapy

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dc.contributor.authorAnilmis, Nur Merve
dc.contributor.authorKara, Goknur
dc.contributor.authorKılıçay, Ebru
dc.contributor.authorHazer, Baki
dc.contributor.authorDenkbas, Emir Baki
dc.date.accessioned2020-05-21T01:27:35Z
dc.date.available2020-05-21T01:27:35Z
dc.date.issued2019
dc.departmentKapadokya Üniversitesi
dc.descriptionTARAMASCOPUS
dc.descriptionTARAMAPUBMED
dc.descriptionTARAMAWOS
dc.description.abstractIn this study, the anticancer activities of two siRNA carriers were compared using a human lung adenocarcinoma epithelial cell line (A549). Firstly, poly(styrene)-graft-poly(linoleic acid) (PS-g-PLina) and poly(styrene)-graft-poly(linoleic acid)-graft-poly(ethylene glycol) (PS-g-PLina-g-PEG) graft copolymers were synthesized by free-radical polymerization. PS-PLina and PS-PLina-PEG nanoparticles (NPs) were prepared by solvent evaporation method and were then characterized. The size was found as 150 +/- 10 nm for PS-PLina and 184 +/- 6 nm for PS-PLina-PEG NPs. The NPs were functionalized with poly(l-lysine) (PLL) for c-myc siRNA conjugation. siRNA entrapment efficiencies were found in the range of 4-63% for PS-PLina-PLL and 6-42% for PS-PLina-PEG-PLL NPs. The short-term stability test was realised for 1 month. siRNA release profiles were also investigated. In vitro anticancer activity of siRNA-NPs was determined by MTT, flow cytometry, and fluorescence microscopy analyses. Obtained findings showed that both NPs systems were promising as siRNA delivery tool for lung cancer therapy.
dc.description.sponsorshipBulent Ecevit University Research FundBulent Ecevit University [BEU-2016-33496813-01]; Kapadokya University [KUN(0).2018-BAGP-001]
dc.description.sponsorshipThis work was financially supported by Bulent Ecevit University Research Fund (Grant no. BEU-2016-33496813-01) and Kapadokya University #KUN(0).2018-BAGP-001.
dc.identifier.doi10.1080/02652048.2019.1665117
dc.identifier.endpage648en_US
dc.identifier.issn0265-2048
dc.identifier.issn1464-5246
dc.identifier.issue7en_US
dc.identifier.pmid31509450
dc.identifier.scopusqualityQ2
dc.identifier.startpage635en_US
dc.identifier.urihttps://doi.org/10.1080/02652048.2019.1665117
dc.identifier.urihttps://hdl.handle.net/20.500.12695/31
dc.identifier.volume36en_US
dc.identifier.wosWOS:000487149700001
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Sceince
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherTaylor & Francis Ltd
dc.relation.ispartofJournal Of Microencapsulation
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectsiRNA
dc.subjectPS-g-PLina
dc.subjectPS-g-PLina-PEG
dc.subjectnanoparticles
dc.subjectlung cancer
dc.titleDesigning siRNA-conjugated plant oil-based nanoparticles for gene silencing and cancer therapy
dc.typeArticle

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