Effect of thymoquinone on NRF2/NF-kB/MAPK pathway in methotrexate-induced rat testis injury

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dc.authorid0000-0002-0917-0378
dc.contributor.authorKaymak, Emin
dc.contributor.authorCeylan, Tayfun
dc.contributor.authorAkin, Ali Tuğrul
dc.contributor.authorKuloğlu, Nurhan
dc.contributor.authorSayan, Meryem
dc.contributor.authorDeğer, Necla
dc.contributor.authorÖnal, Esra
dc.contributor.authorYıldırım, Ayşegül Burçin
dc.contributor.authorKarabulut, Derya
dc.date.accessioned2024-09-16T11:39:50Z
dc.date.available2024-09-16T11:39:50Z
dc.date.issued2024en_US
dc.departmentKapadokya Üniversitesi, Diş Hekimliği Fakültesi, Diş Hekimliği Bölümü
dc.description.abstractObjective(s): In this study, we aimed to investigate the protective effect of Thymoquinone (THQ) against testicular damage caused by Methotrexate (MTX). Materials and Methods: This study consists of 5 groups: Control, Olive oil, THQ, MTX, and MTX+THQ. At the end of the experiment, spermiogram analysis was performed on the rats. In addition, testicular tissues were taken and histopathology, immunohistochemistry, and biochemistry analysis were performed. Biochemical analyses were performed on the serums. Results: According to the results obtained, spermiogram values, Johnson’s testicular biopsy score, SOD, CAT, GPx, FSH, LH, and testosterone values were statistically significantly decreased in the MTX group compared to the control group. In the MTX+THQ group, spermiogram values, Johnson’s testicular biopsy score, SOD, CAT, GPx, FSH, LH, and testosterone values increased statistically significantly compared to the MTX group. NRF2 and HO-1 immunoreactivity were statistically significantly decreased in the MTX group compared to the control group. In the MTX+THQ group, NRF2 and HO-1 immunoreactivity were statistically significantly increased compared to the MTX group. The level of MDA, which is important in lipid damage, and the level of biochemistry results of TNF-?, IL1-?, and IL-6, which are important markers, and the results of p-NF-kB and P38 immunoreactivity were statistically significantly increased in the MTX group compared to the control group. In the MTX+THQ group, these parameters showed a significant decrease compared to the MTX group. Conclusion: According to these results, it is thought that THQ will play a protective role against infertility caused by chemotherapy-induced testicular damage.
dc.identifier.citationKAYMAK, E., CEYLAN, T., AKİN, A. T., KULOĞLU, N., SAYAN, M., DEĞER, N., … KARABULUT, D. (2024). Effect of thymoquinone on NRF2 NF-kB MAPK pathway in methotrexate-induced rat testis injury. Iranian Journal of Basic Medical Sciences, 27(11), 1410–1416.
dc.identifier.endpage1416en_US
dc.identifier.issue11en_US
dc.identifier.startpage1410en_US
dc.identifier.urihttps://ijbms.mums.ac.ir/article_24663.html
dc.identifier.urihttps://doi.org/10.22038/ijbms.2024.77714.16813
dc.identifier.urihttps://hdl.handle.net/20.500.12695/2858
dc.identifier.volume27en_US
dc.institutionauthorCeylan, Tayfun
dc.language.isoen
dc.publisherIranian Journal of Basic Medical Sciences
dc.relation.ispartofIranian Journal of Basic Medical Sciences
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectInflammation
dc.subjectMethotrexate
dc.subjectOxidative stress
dc.subjectTestis
dc.subjectThymoquinone
dc.titleEffect of thymoquinone on NRF2/NF-kB/MAPK pathway in methotrexate-induced rat testis injury
dc.typeArticle

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