Evaluation Of Epididymitis Tissue Of Cisplatin-Induced Rats

Abstract

As a chemotherapeutic agent, cisplatin is an antineoplastic agent used in the treatment of various solid tumors such as lung, testicle and bladder cancer (Ezaki, Nishiumi, Azuma, & Yoshida, 2017; Salem, Helmi, & Assaf, 2018). Autophagy abnormality is one of the mechanisms of cell death leading to the development of diseases such as cancer (Yang & Klionsky, 2010). In this study, it was aimed to determine the damage caused by cisplatin on tissue by using autophagy inhibitor and activator. A total of 24 Wistar albino male rats were used in the study, 6 animals in each group. Group I; Control, Group II; Cisplatin (8 mg / kg), Group III; Rapamycin (2 mg / kg), Group IV; Group of 3-methylated (15 mg / kg). While rapamycin and 3-methyladenine were administered for 15 days, cisplatin was applied to these groups on the 7th day of a single experiment. At the end of the experiment, the organs of the rats, which were anesthetized, were removed and placed in formaldehyde for histological follow-up. Hematoxylin-Eosin and Heat-shock protein70 (HSP70) immunohistochemistry was applied to the sections taken after histological techniques. Group I tissue sections had a normal histological appearance. In Group II sections, some areas were disintegrated in the tubular basement membrane and vacuolization was observed in the tubule. In addition, some epithelial cells were observed to be more eosinophilic. Tissue sections of groups III and IV had a more regular appearance as epithelialization, tubule basement membrane. Eosinophilic cells were observed in tubular epithelialization of group IV. HSP70 immunoreactivity was observed in the inter-tubular connective tissue of all groups. HSP70 immunoreactivity was observed in the tubular epithelium of both Group III and Group IV tissue sections, mostly in Group II. Sperm transport, maturation and storage is the most important task of the epididymis (Wang & Kumar, 2012). It was concluded that the organ was affected by agents such as cisplatin to maintain semen quality and potentiality of spermatozoa. We think that HSP70 mediates the formation of autophagy that occurs in the organ.