Recombinant expression and coexpression of oyster defensin and proline-rich peptide in Komagataella phaffii

Basit Öğe Kaydı
dc.authorid0000-0002-8724-0466
dc.contributor.authorErdem Büyükkiraz, Mine
dc.contributor.authorKesmen, Zülal
dc.date.accessioned2022-02-16T06:43:48Z
dc.date.available2022-02-16T06:43:48Z
dc.date.issued2021en_US
dc.departmentKapadokya Üniversitesi, Sağlık Bilimleri Yüksekokulu, Beslenme ve Diyetetik Bölümü
dc.descriptionTARAMAWOS
dc.descriptionTARAMAPUBMED
dc.descriptionTARAMASCOPUS
dc.description.abstractProline-rich peptide (CgPrp) and defensin (CgDef), oyster (Crassostrea gigas)-originated antimicrobial peptides (AMPs), were produced by the recombinant technique in Komagataella phaffii GS115 cells. For this purpose, the nucleotide sequences encoding the CgPrp and CgDef peptides were synthesized by the recursive PCR technique, and ligated in pPICZaA expression vector. Additionally, the expression cassettes of pPICZ?A-CgDef and pPICZ?A-CgPrp were combined using in vitro multimer ligation strategy to construct the coexpression vector pPICZaA-CgPrp-CgDef. The expression and coexpression vectors transformed into K. phaffii GS115 cells by electroporation. At the end of the 0.5% methanol-induced expression stage for 96 h, the recombinant peptides were purified from the culture medium. The concentrations of purified peptides were changed between 1.05 and 1.21 mg/L. The recombinant peptides successfully inhibited the growth of tested Gram-positive bacterial strains belonging to Staphylococcus aureus, methicillin-resistant S. aureus (MRSA), Listeria monocytogenes, and Bacillus cereus. The minimum inhibitory concentrations (MIC) of recombinant CgPrp, CgDef, and CgPrp-CgDef peptides against tested bacteria were in the range of 12.50–25.00, 18.75–75.00, and 5.80–11.60 pg/?l, respectively. The results of the study proved that the recombinant CgPrp, CgDef, and CgPrp-CgDef peptides expressed in K. phaffii might have good potential for the inhibition of common Gram-positive pathogenic bacteria, including drug-resistant MRSA.
dc.identifier.doi10.1002/bab.2262
dc.identifier.endpage10en_US
dc.identifier.issue00en_US
dc.identifier.scopusqualityQ2
dc.identifier.startpage1en_US
dc.identifier.urihttps://doi.org/10.1002/bab.2262
dc.identifier.urihttps://hdl.handle.net/20.500.12695/1490
dc.identifier.wosWOS:000706395200001
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Sceince
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorErdem Büyükkiraz, Mine
dc.language.isoen
dc.publisherBiotechnology and Applied Biochemistry
dc.relation.ispartofBiotechnology and Applied Biochemistry
dc.relation.publicationcategoryMakale - Ulusal Hakemli Dergi - Başka Kurum Yazarı
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectantimicrobial peptide
dc.subjectdefensin
dc.subjectexpression
dc.subjectKomagataella phaffii
dc.subjectmultimerligation
dc.subjectproline-rich peptide
dc.subjectCrassostrea gigas
dc.titleRecombinant expression and coexpression of oyster defensin and proline-rich peptide in Komagataella phaffii
dc.typeArticle

Dosyalar

Orijinal paket
Listeleniyor 1 - 1 / 1
[ X ]
İsim:
Biotech and App Biochem - 2021 - Erdem B y kkiraz - Recombinant expression and coexpression of oyster defensin and.pdf
Boyut:
1.33 MB
Biçim:
Adobe Portable Document Format
Açıklama:
Makale / Article
İndir
Lisans paketi
Listeleniyor 1 - 1 / 1
[ X ]
İsim:
license.txt
Boyut:
1.44 KB
Biçim:
Item-specific license agreed upon to submission
Açıklama:
İndir

Koleksiyon

Beslenme ve Diyetetik - Makale Koleksiyonu