Comparison of the Acute and Cumulative Dose Administrations in Doxorubicin-induced Hepatotoxicity via Evaluation of the Histopathological Changes and Inflammation in Rats
Yükleniyor...
Tarih
2020
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Experimental and Applied Medical Science
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Doxorubicin (DOX) may lead to hepatotoxicity when administered chronically or in a high dose. The aim of this study is to determine dose–dependent effects of DOX in rat liver tissue. Thirty male Wistar albino rats were divided into three groups; group I as control, group II as receiving chronically DOX (2 mg/kg, twice in a week, total 20 mg/kg, intraperitoneally) and group III as receiving an acute–single dose of DOX (15 mg/kg, intraperitoneally, on the 20th day) administered groups. At the end of 30th day, animals were sacrificed, and liver tissues were extracted for histopathological and immunohistochemical evaluation. Sections were stained with hematoxylin & eosin to evaluate the histopathological changes and TNF-? and IL-6 expressions were detected by immunohistochemical staining. Both chronic and acute administrations of DOX triggered a significant liver damage. However, it was observed that liver damage induced by acute–single dose DOX administrations were higher than those induced by chronic DOX administrations. TNF-? and IL-6 immunoreactivity was significantly increased in both group II and III group compared to control group. However, immunoreactivity of TNF-? was substantially higher in the group III compared to control. These results demonstrated that acute administrations of DOX relatively induce serious liver damage and inflammatory response.
Açıklama
Anahtar Kelimeler
Doxorubicin, Hepatotoxicity, Inflammation, Liver damage
Kaynak
Experimental and Applied Medical Science
WoS Q Değeri
Scopus Q Değeri
Cilt
1
Sayı
3
Künye
Ali, A. K. I. N., Kaymak, E., Karabulut, D., Doğanyiğit, Z., Ceylan, T., Toluk, A., & Özdamar, S. Comparison of the acute and cumulative dose administrations in doxorubicin-induced hepatotoxicity via evaluation of the histopathological changes and inflammation in rats. Experimental and Applied Medical Science, 1(3), 73-81.
