Isolation and characterization a novel Acinetobacter bacteriophage with activity against several multidrug-resistant Gram-negative bacteria

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dc.authoridhttps://orcid.org/0000-0002-5746-7053
dc.authoridhttps://orcid.org/0000-0003-3243-6338
dc.authoridhttps://orcid.org/0000-0003-4279-434X
dc.contributor.authorHamzeli, Nur
dc.contributor.authorAktaş, Ahmet
dc.contributor.authorKöksal Çakırlar, Fatma
dc.date.accessioned2025-11-21T08:38:23Z
dc.date.available2025-11-21T08:38:23Z
dc.date.issued06/2025
dc.departmentKapadokya Üniversitesi, Kapadokya Meslek Yüksekokulu, Tıbbi Laboratuvar Teknikleri Bölümü
dc.description.abstractAcinetobacter baumannii (A. baumannii) is a notorious nosocomial pathogen that is frequently associated with multidrug resistance around the world. Endolysin is a bacteriophage-produced hydrolytic enzyme. In this study, an urban waste water sample (1 L) was collected from Istanbul. A double-agar plating technique was used for host range analysis. The purified genomic DNA sequences were performed on the Miseq. De novo assembly of the crude sequences yielded a double-stranded DNA molecule with a length of 45,679 bp and a guanine-cytosine content of 37.6%. Genome annotation revealed that the vB_AbaM_YNAF genome comprises 85 open reading frames, 23 of which are functional and do not define any tRNA-rRNA genes. Electron microscopy examination and phylogenetic analysis of the genome revealed that vB_AbaM_YNAF represents a novel origin of an unclassified Obolenskvirus belonging to the class Caudoviricetes. vB_AbaM_YNAF infected one reference and three different multidrug-resistant (MDR) strains of each of A. baumannii and K. pneumoniae. However, it did not have any effect on a reference E. coli strain. Based on all of these findings, LysYAN could be a potential agent for treating MDR-Gram-negative bacteria. Further investigations on vB_AbaM_YNAF may be beneficial for designing an alternative weapon with probable wide host-range activity to fight MDR infections.
dc.identifier.citationİşlek, Y., Hamzeli, N., Aktaş, A., & Köksal Çakırlar, F. (2025). Isolation and characterization of a novel Acinetobacter bacteriophage with activity against several multidrug‑resistant Gram‑negative bacteria. Microbial Pathogenesis, 203, 107488. https://doi.org/10.1016/j.micpath.2025.107488
dc.identifier.doi10.1016/j.micpath.2025.107488
dc.identifier.endpage11
dc.identifier.issue107488
dc.identifier.scopusqualityQ2
dc.identifier.startpage1
dc.identifier.urihttps://hdl.handle.net/20.500.12695/3848
dc.identifier.volume203
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.institutionauthorİşlek, Yelda
dc.institutionauthoridhttps://orcid.org/0000-0003-3459-011X
dc.language.isoen
dc.publisherYelda İşlek
dc.relation.ispartofMicrobial Pathogenesis
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.subjectAcinetobacter baumannii
dc.subjectBacteriophage
dc.subjectEndolysin
dc.subjectObolenskvirus
dc.subjectMultidrug resistance
dc.subjectHolin
dc.titleIsolation and characterization a novel Acinetobacter bacteriophage with activity against several multidrug-resistant Gram-negative bacteria
dc.typeArticle

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