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Ameliyathanede Cerrahi Örnek (Spesmen) Yönetimi
(Kongre Kitabevi, 2018) Ceylan, Tayfun
Ameliyathanede yönetim organizasyon ve cerrahi uygulamalar
Anti-inflammatory and anti-apoptotic effects of naringin on bacterial endotoxin-induced small intestine damage in rats
(2022) Akin, Ali Tuğrul; El Bechir, Mohamed Lemine; Kaymak, Emin; Ceylan, Tayfun; Sayan, Meryem; Değer, Necla; Karabulut, Derya; Toluk, Ayşe
Purpose: The aim of this study is to investigate the anti inflammatory and anti-apoptotic effects of naringin (NRG), which has many biological properties, on bacterial endotoxin-induced small intestine damage in rats. Materials and Methods: For this purpose, 40 female Wistar albino rats were divided into 4 groups as Control (group given no treatment), LPS (group given 10 mg/kg/i.p lipopolysaccharide), NRG (group given 100 mg/kg/i.p naringin for 14 days) and LPS + NRG (group given 100 mg/kg/i.p naringin for 14 days before 10 mg/kg/i.p lipopolysaccharide injection). After experimental procedure, small intestine tissues of animals were extracted and prepared according to tissue processing protocol. Hematoxylin and Eosin staining were performed to evaluate the histopathological changes and histological damage scoring was applied to compare experimental groups in terms of histopathological changes. Moreover, TNF-? and Caspase-3 expression levels were detected by immunohistochemical staining and the density of immunoreactivity were scored to determine the difference in the expression levels of TNF-? and Caspase-3 expressions among groups. Results: Epithelial and Brunner’s gland damage, mononuclear cell infiltration, hemorrhage, and TNF-? and Caspase-3 expressions significantly increased in the LPS group. However, NRG administrations exerted a strong protective effect on the small intestine tissues in terms of these parameters in LPS+NRG group. Conclusion: This study demonstrated that 100 mg/kg NRG injection can be regarded as a protective agent against negative effects of endotoxin-induced infection on the intestinal mucosa and that it should not be disregarded in further clinical trials.
Chloroquine attenuates chronic hypoxia-induced testicular damage via suppressing endoplasmic reticulum stress and apoptosis in experimental rat model
(Wiley Online Library, 2022) Akin, Ali Tuğrul; Kaymak, Emin; Ceylan, Tayfun; Öztürk, Emel; Başaran, Kemal Erdem; Karabulut, Derya; Özdamar, Saim; Yakan, Birkan
Chronic hypoxia negatively affects male fertility by causing pathological changes in male reproductive system. However, underlying mechanisms of this damage are unknown. Chloroquine (CLQ) is an anti-inflammatory agent that is widely used in the treatment of inflammation-related diseases such as malaria and rheumatoid arthritis. This study aimed to investigate the therapeutic effects of CLQ in the hypoxiainduced testicular damage via assessment of hypoxic response, endoplasmic reticulum stress and apoptosis. For this purpose, 32 Wistar albino rats were divided into 4 groups as control (given 20%-21% O2, no treatment), CLQ (given 50 mg/kg and 20%-21% O2 for 28 days), hypoxia (HX) (given 10% O2 for 28 days) and HX + CLQ (given 50 mg/kg and 10% O2 for 28 days). After the experiment, blood samples and testicular tissues were taken. Histopathological evaluation was performed on testicular tissues and hypoxia-inducible factor 1-? (HIF1-?), heat shock proteins (HSPs) HSP70, HSP90 and growth arrest and DNA damage-inducible gene 153 (GADD153) expression levels were detected via immunohistochemistry. Moreover, apoptotic cells were detected via terminal deoxynucleotidyl transferase dUTP nick end labelling (TUNEL) staining and serum testosterone levels were determined by enzyme-linked immunosorbent assay (ELISA) assay. Histopathological changes, apoptotic cell numbers and HIF1-?, HSP70, HSP90 and GADD153 expressions significantly increased in HX group (P < .05). Moreover, serum testosterone levels decreased in this group (P > .05). However, CLQ exerted a strong ameliorative effect on all parameters in HX + CLQ group. According to our results, we suggested that CLQ can be considered as an alternative protective agent for eliminating the negative effects of hypoxic conditions on male fertility.
Comparison of the Acute and Cumulative Dose Administrations in Doxorubicin-induced Hepatotoxicity via Evaluation of the Histopathological Changes and Inflammation in Rats
(Experimental and Applied Medical Science, 2020) Akin, Ali Tuğrul; Kaymak, Emin; Karabulut, Derya; Doğanyiğit, Züleyha; Ceylan, Tayfun; Toluk, Ayşe; Özdamar, Saim
Doxorubicin (DOX) may lead to hepatotoxicity when administered chronically or in a high dose. The aim of this study is to determine dose–dependent effects of DOX in rat liver tissue. Thirty male Wistar albino rats were divided into three groups; group I as control, group II as receiving chronically DOX (2 mg/kg, twice in a week, total 20 mg/kg, intraperitoneally) and group III as receiving an acute–single dose of DOX (15 mg/kg, intraperitoneally, on the 20th day) administered groups. At the end of 30th day, animals were sacrificed, and liver tissues were extracted for histopathological and immunohistochemical evaluation. Sections were stained with hematoxylin & eosin to evaluate the histopathological changes and TNF-? and IL-6 expressions were detected by immunohistochemical staining. Both chronic and acute administrations of DOX triggered a significant liver damage. However, it was observed that liver damage induced by acute–single dose DOX administrations were higher than those induced by chronic DOX administrations. TNF-? and IL-6 immunoreactivity was significantly increased in both group II and III group compared to control group. However, immunoreactivity of TNF-? was substantially higher in the group III compared to control. These results demonstrated that acute administrations of DOX relatively induce serious liver damage and inflammatory response.
Crocin Suppresses Inflammatory Response in LPS-Induced Acute Lung Injury (ALI) Via Regulation of HMGB1/TLR4 Inflammation Pathway
(DergiPark, 2024) Ceylan, Tayfun; Akin, Ali Tuğrul; Kaymak, Emin; Varinli, Şaban; Toluk, Ayşe
Background and Purpose: The most significant pathogen hypothesized to be causing the formation of Acute lung injury (ALI) in sepsis is thought to be lipopolysaccharide (LPS), a key endotoxin component of gram-negative bacteria. The main objective of this study is to determine possible anti-inflammatory effects of crocin (CRO) which has many biological properties such as anti-inflammatory, antioxidant, and anti-apoptotic in LPS-induced ALI. Methods: 40 Wistar albino rats were divided into four groups: Control (no treatment), CRO (given 50 mg/kg crocin for 9 days), LPS (given 30 mg/kg LPS at 9th day), LPS+CRO (given 50 mg/kg crocin for 9 days and 30 mg/kg LPS at 9th day). After experimental, rats were sacrificed and lungs were extracted. Histological examinations were performed in the lung tissue and the changes in the HMGB1 and TLR4 expressions were determined via immunohistochemical staining. Results and Conclusion: Hemorrhage, mononuclear cell infiltration and HMGB1 and TLR4 expressions significantly increased in the LPS group. However, CRO administrations exerted a strong protective effect on the lungs in terms of these parameters in LPS+CRO group. According to our results, we suggest that CRO can be considered as a protective agent against LPS induced ALI via inhibition of HMGB1/TLR4 pathway-mediated inflammatory response.
Crocin Suppresses Inflammatory Response in LPS-Induced Acute Lung Injury (ALI) Via Regulation of HMGB1/TLR4 Inflammation Pathway
(2022) Akin, Ali Tuğrul; Ceylan, Tayfun; Kaymak, Emin
Acute lung injury (ALI) is a frequent consequence which has high morbidity and mortality in sepsis. The most significant pathogen hypothesized to be causing the formation of ALI in sepsis is thought to be lipopolysaccharide (LPS), a key endotoxin component of gram-negative bacteria. Although endotoxin-induced inflammation is a complex process, it can be artificially produced via administration of lipopolysaccharide (LPS) in Experimental sepsis models. The main objective of thisstudy isto determine possible anti-inflammatory effects of crocin (CRO) which has many biological properties such as anti-inflammatory, antioxidant, and anti-apoptotic in LPS-induced ALI. For this purpose, total 40 Wistar albino rats randomly divided into four groups, ten rats in per group: Control (no treatment), CRO (given 50 mg/kg crocin for 9 days), LPS (given 30 mg/kg LPS at 9th day), LPS+CRO (given 50 mg/kg crocin for 9 days and 30 mg/kg LPS at 9th day). After experimental protocol, rats were sacrificed and lung tissues were extracted for further analysis. Histological examinations were performed for detecting histopathological changes in the lung tissue and the changes in the HMGB1 and TLR4 expressions were determined via immunohistochemical staining. Hemorrhage, mononuclear cell infiltration and HMGB1 and TLR4 expressions significantly increased in the LPS group (p<0.05). However, CRO administrations exerted a strong protective effect on the lung tissues in terms of these parameters in LPS+CRO group (p<0.05). According to our results, we suggest that CRO can be considered as a protective agent against bacterial endotoxin induced ALI via inhibition of HMGB1/TLR4 pathway-mediated inflammatory response.
Difüzyon Tensör Görüntüleri ile Traktografi Yapılarak Beyin İçerisindeki Bazı Liflerin 3 Boyutlu Oluşturulması ve Anatomi Eğitimine Katkısı
(2019) Yiğit, Hüseyin; Ceylan, Tayfun; Acer, Niyazi
Giriş ve Amaç: Difüzyon Tensör Görüntüleme ile dokulardaki difüzyonun hangi yönde kısıtlandığı niceliksel olarak ifade edilebilmektedir. Bu niceliksel ifadenin yanı sıra, fiber traktografi algoritmalarının kullanılmasıyla da, beyindeki ak madde 3 boyutlu olarak gösterilebilmektedir. Bu çalışmada DtiStudio programı ile traktografi yapılarak, tractus corticospinalis, forceps major ve minor, fasciculus arcuatus ve uncinatus, fasciculus longitudinalis superior ve inferior gibi traktların ortaya çıkarılması ve bu traktların MRIcroGL adlı programı ile beyin içerisinde 3 boyutlu olarak gösterilmesi amaçlanmıştır. Gereç ve Yöntem: Görüntüler, DTI mapping menüsü ile traktografiye hazır hale getirilir. Elde edilen dosya, Fiber tracking menüsü kullanılarak; aksiyal, koronal ve sagittal kesitlerin üzerinde, istenen bölgenin seçilmesine olanak sağlar. Ardından bu 3 kesitten ilgili traktusun başlangıç (ROI-1) ve sonlanış noktası (ROI-2) seçilerek, traktus 3 boyutlu olarak elde edilir. Elde edilen bu traktuslar, mricrogl programı yardımı ile şablonlar kullanılarak, beyin içerisine yerleştirilir. Tartışma ve Sonuç: Literatürde farklı yöntemler kullanılarak fiber traktografi yapılabilmektedir. Ancak klinik bilgiler ile doğruluğu onaylanan ilk traktografi algoritması FACT (Fiber Assignment by Continuous Tracking)’tir. Bu çalışma ile FACT algoritması kullanan Dtistudio kullanılarak bazı traktlar gösterilmiştir. Bu tip programların hem eğitim hem de akademik alanlarda kullanımının yaygınlaşmasının, beyin anatomisinin anlaşılmasında ve klinik problemlere katkı sunmada yararlı olacağı kanaatindeyiz.
Efects of pseudoephedrine on rat fetal bone development: evaluation by three diferent methods
(Springer, 2023) Yiğit, Hüseyin; Balcıoğlu, Esra; Uçar, İlyas; Değermenci, Muhammet; Önder, Gözde Özge; Ceylan, Tayfun; Unur, Erdoğan
Pseudoephedrine (PSE) is an agent that is contained in common cold medications. The agent, which is used to treat cold and cough, is the fourth most prescribed drug group in some countries. During pregnancy, expectant mothers use PSE for colds and other reasons. One out of every four expectant mothers use PSE alone or in combination with other medicines for various reasons. This study was aimed to investigate efects of PSE on long bones development in rat during fetal growth. Pregnant rats were divided into fve groups: control and four experimental groups (25 mg/kg, 50 mg/kg,100 mg/kg, 200 mg/kg PSE). Between 1 and 20 days of pregnancy, PSE was given to them by gavage. Weights and heights of fetuses isolated by cesarean on the 21st day were measured. Ossifcation of femur and humerus was examined by three diferent methods mentioned earlier. Depending on the dose increase, all morphometric data, ossifcation rate and bone length of the fetuses were decreased. Besides, it was determined that the amount of Calcium in the bone tissue decreased in the analyzes made with SEM–EDX Analysis. The data obtained from this study reveal that the use of PSE during pregnancy disrupts the exist ing balance in the bone and negatively afects ossifcation due to the dose increase. In conclusion, we present descriptive and novel data on the efects of PSE use during pregnancy on the bone development of rat fetal long bones.
Effect of Chloroquine in Chronic Hypoxia-Induced Bowel Damage
(20. Ulusal Anatomi Kongresi, 2019) Kaymak, Emin; Akin, Ali Tuğrul; Yalçın, Betül; Öztürk, Emel; Ceylan, Tayfun; Başaran, Kemal Erdem; Karabulut, Derya; Doğanyiğit, Züleyha; Özdamar, Saim; Yakan, Birkan
Effect of chloroquine in chronic hypoxia-induced bowel damage
Effect of thymoquinone on NRF2/NF-kB/MAPK pathway in methotrexate-induced rat testis injury
(Iranian Journal of Basic Medical Sciences, 2024) Kaymak, Emin; Ceylan, Tayfun; Akin, Ali Tuğrul; Kuloğlu, Nurhan; Sayan, Meryem; Değer, Necla; Önal, Esra; Yıldırım, Ayşegül Burçin; Karabulut, Derya
Objective(s): In this study, we aimed to investigate the protective effect of Thymoquinone (THQ) against testicular damage caused by Methotrexate (MTX). Materials and Methods: This study consists of 5 groups: Control, Olive oil, THQ, MTX, and MTX+THQ. At the end of the experiment, spermiogram analysis was performed on the rats. In addition, testicular tissues were taken and histopathology, immunohistochemistry, and biochemistry analysis were performed. Biochemical analyses were performed on the serums. Results: According to the results obtained, spermiogram values, Johnson’s testicular biopsy score, SOD, CAT, GPx, FSH, LH, and testosterone values were statistically significantly decreased in the MTX group compared to the control group. In the MTX+THQ group, spermiogram values, Johnson’s testicular biopsy score, SOD, CAT, GPx, FSH, LH, and testosterone values increased statistically significantly compared to the MTX group. NRF2 and HO-1 immunoreactivity were statistically significantly decreased in the MTX group compared to the control group. In the MTX+THQ group, NRF2 and HO-1 immunoreactivity were statistically significantly increased compared to the MTX group. The level of MDA, which is important in lipid damage, and the level of biochemistry results of TNF-?, IL1-?, and IL-6, which are important markers, and the results of p-NF-kB and P38 immunoreactivity were statistically significantly increased in the MTX group compared to the control group. In the MTX+THQ group, these parameters showed a significant decrease compared to the MTX group. Conclusion: According to these results, it is thought that THQ will play a protective role against infertility caused by chemotherapy-induced testicular damage.
Effect of vitamin B12 on methotrexate-induced cardiotoxicity in rats
(Iranian Journal of Basic Medical Sciences, 2024) Kuloğlu, Nurhan; Karabulut, Derya; Kaymak, Emin; Akin, Ali Tuğrul; Ceylan, Tayfun; Yıldırım, Ayşegül Burçin; Yakan, Birkan
Objective(s): Methotrexate (MTX) is a drug with anti-inflammatory and immunosuppressive effects and is also a folic acid antagonist. Our aim in this study is to determine the molecular mechanisms of cardiotoxicity caused by MTX, a chemotherapeutic drug, and to evaluate the protective effects of vitamin B12 on this toxicity. Materials and Methods: A total of 32 rats were used in our study and 4 groups were formed. Control group, Vit B12 group (3 ?g/kg B12 for 15 days, IP), MTX group (20 mg/kg MTX single dose on day 8 of the experiment, IP), MTX +Vit B12 group (3 ?g/kg, IP ), Vit B12 throughout the 15 days, and a single dose of 20 mg/kg MTX (IP) on day 8 of the experiment. Immunohistochemically, expressions of hypoxia-inducible factor 1? (HIF1-?), vascular endothelial growth factor receptor-2 (VEGFR-2), erythropoietin (EPO), and interleukin-6 (IL-6) were evaluated in the heart tissue. Total catalase (CAT), superoxide dismutase (SOD), and malondialdehyde (MDA) levels were measured in the heart tissue. At the same time, ANP and NT-proBNP levels were measured in the blood serum. Results: In the study, the expression of HIF1-? and VEGFR-2 increased significantly in the MTX group, while IL-6 and EPO significantly decreased. At the same time, CAT and SOD levels were significantly decreased and MDA levels increased significantly in the MTX group. While vitamin B12 significantly corrected all these values, it also greatly reduced the increases in ANP and NT-proBNP levels caused by MTX. Conclusion: It is important to use Vit B12 before and after MTX administration to replace the folate that MTX has reduced.
Effects of pseudoephedrine on rat fetal bone development: evaluation by three different methods
(SPRINGER, 2023) Yiğit, Hüseyin; Balcıoğlu, Esra; Uçar, İlyas; Değermenci, Muhammet; Önder, Gözde Özge; Ceylan, Tayfun; Unur, Erdoğan
Pseudoephedrine (PSE) is an agent that is contained in common cold medications. The agent, which is used to treat cold and cough, is the fourth most prescribed drug group in some countries. During pregnancy, expectant mothers use PSE for colds and other reasons. One out of every four expectant mothers use PSE alone or in combination with other medicines for various reasons. This study was aimed to investigate effects of PSE on long bones development in rat during fetal growth. Pregnant rats were divided into five groups: control and four experimental groups (25 mg/kg, 50 mg/kg, 100 mg/kg, 200 mg/kg PSE). Between 1 and 20 days of pregnancy, PSE was given to them by gavage. Weights and heights of fetuses isolated by cesarean on the 21st day were measured. Ossification of femur and humerus was examined by three different methods mentioned earlier. Depending on the dose increase, all morphometric data, ossification rate and bone length of the fetuses were decreased. Besides, it was determined that the amount of Calcium in the bone tissue decreased in the analyzes made with SEM–EDX Analysis. The data obtained from this study reveal that the use of PSE during pregnancy disrupts the existing balance in the bone and negatively affects ossification due to the dose increase. In conclusion, we present descriptive and novel data on the effects of PSE use during pregnancy on the bone development of rat fetal long bones.
Evaluation Of Epididymitis Tissue Of Cisplatin-Induced Rats
(SANAR, 2020) Ceylan, Tayfun; Karabulut, Derya; Öztürk, Emel; Akin, Ali Tuğrul; Kaymak, Emin; Yakan, Birkan
As a chemotherapeutic agent, cisplatin is an antineoplastic agent used in the treatment of various solid tumors such as lung, testicle and bladder cancer (Ezaki, Nishiumi, Azuma, & Yoshida, 2017; Salem, Helmi, & Assaf, 2018). Autophagy abnormality is one of the mechanisms of cell death leading to the development of diseases such as cancer (Yang & Klionsky, 2010). In this study, it was aimed to determine the damage caused by cisplatin on tissue by using autophagy inhibitor and activator. A total of 24 Wistar albino male rats were used in the study, 6 animals in each group. Group I; Control, Group II; Cisplatin (8 mg / kg), Group III; Rapamycin (2 mg / kg), Group IV; Group of 3-methylated (15 mg / kg). While rapamycin and 3-methyladenine were administered for 15 days, cisplatin was applied to these groups on the 7th day of a single experiment. At the end of the experiment, the organs of the rats, which were anesthetized, were removed and placed in formaldehyde for histological follow-up. Hematoxylin-Eosin and Heat-shock protein70 (HSP70) immunohistochemistry was applied to the sections taken after histological techniques. Group I tissue sections had a normal histological appearance. In Group II sections, some areas were disintegrated in the tubular basement membrane and vacuolization was observed in the tubule. In addition, some epithelial cells were observed to be more eosinophilic. Tissue sections of groups III and IV had a more regular appearance as epithelialization, tubule basement membrane. Eosinophilic cells were observed in tubular epithelialization of group IV. HSP70 immunoreactivity was observed in the inter-tubular connective tissue of all groups. HSP70 immunoreactivity was observed in the tubular epithelium of both Group III and Group IV tissue sections, mostly in Group II. Sperm transport, maturation and storage is the most important task of the epididymis (Wang & Kumar, 2012). It was concluded that the organ was affected by agents such as cisplatin to maintain semen quality and potentiality of spermatozoa. We think that HSP70 mediates the formation of autophagy that occurs in the organ.
Gama Işınlarına Maruz Kalan Sıçanlarda Myricitrin ve Chebulinic Asitin Böbrek Üzerine Etkilerinin Stereolojik, Histokimyasal Yöntemlerle Belirlenmesi
(Nuh Naci Yazgan Ulusal Sağlık Bilimleri Kongresi, 2024) Alkan, Işınsu; Bozkurt, Özlem; Bozkurt, Ali; Ceylan, Tayfun; Altunkaynak, Berrin Zuhal
Amaç: Bu çalışmada Gama radyasyonuna maruz bırakılan sıçanlarla; tedavi amacı ile antioksidan madde uygulanan sıçanların böbrek dokuları kullanılarak Gama radyasyonunun böbrek fonksiyonları üzerine etkilerinin ve ilerlediği yolakların incelenmesi ile antioksidanlar olan myricitrin ve chebulinic asitin Gama radyasyonunun oluşturduğu zararı önlemedeki etkilerinin açığa çıkarılması amaçlanmıştır. Materyal-Metod: Çalışma kapsamında Control, Sham, Myricitrin, Chebulunic asit, Gama, Gama+Chebulunic asit ve Gama+Myricitrin olamak üzere rastgele olarak 6 gruba ayrılmış 8-10 haftalık 36 Wistar albino cinsi yetişkin erkek sıçanlar’dan (250-300 g) böbrek dokuları temin edilmiştir. Elde edilen böbrek dokuları dereceli alkol, ksilen ve parafin ile muamele edilerek doku takibi gerçekleştirilmiş ve elde edilen böbrek dokuları parafin içerisine gömülmüştür. Bloklanmış dokulardan mikrotom (Leica RM2125RT) yardımı ile 10 mikronluk kesitler alınmış ve hematoksilen-eozin ile doku boyaması gerçekleştirilmiştir. Analiz için hazır hale getirilen örneklerden kamera ataçmanlı mikroskop (Zeiss Primostar 3) ile görüntüler alınmıştır. Alınan görüntüler üzerinde hem AxioCam programı hemde Image J programı üzerinde hacim analizleri gerçekleştirilmiştir. Bulgular: Çalışma kapsamında gerçekleştirilen hacimsel analizlerde korteks hacminde gruplar arasında fark izlenmemiştir. Histopatolojik değerlendirmede ise Gama, Myr ve Che gruplarında böbrek yapısında bozulmalar izlenmiştir. Gama maruziyetinin ardından antioksidan tedavisi gerçekleştirilmiş Gama+Myr, Gama+Che gruplarında ise Kont grubuna benzer sağlıklı morfoloji izlenmiştir. Sonuç: Elde edilen bulgular antioksidanların Gama radyasyonun böbrek üzerinde yarattığı olumsuz etkiler üzerinde koruyucu etkiye sahip olduğunu göstermektedir.
Histological evaluation of the effects of rapamycin and 3-methyladenine on cisplatin-induced epididymal injury in rats
(Cukurova Medical Journal, 2021) Ceylan, Tayfun; Karabulut, Derya; Öztürk, Emel; Akin, Ali Tuğrul; Kaymak, Emin; Yakan, Birkan
Purpose: In this study, we aimed to determine the effects of autophagy inhibitor and activator on Cisplatin (Cis)-induced tissue damage. Materials and Methods: A total of 24 male Wistar albino rats were divided into 4 groups including 6 rats per group in this study. Groups are as follows; Control, Cisplatin (Cis) (8 mg/kg), Rapamycin (Rapa) (2 mg/kg), 3-methyladenine (3-MA) (15 mg/kg). Rapa and 3-MA were given intraperitoneally for 15 days. Cis was administered as a single dose on the 7th day of the experimental period. At the end of the experimental procedure, epididymis tissues were extracted. Hematoxylin and eosin staining and Heat shock protein-70 (HSP70) immunohistochemistry were applied to the sections taken after histological techniques. Results: Dispersion in the tubule basement membrane and vacuolization in the tubule was observed in the Cis group. It was also observed that some epithelial cells were more eosinophilic in the Cis group. Tissue sections of Rapa and 3-MA had a more regular appearance in terms of epithelization and tubule basement membrane. HSP70 immunoreactivity was observed in the intertubuler connective tissue of all groups. Conclusion: The epididymis was affected by agents such as Cis in terms of the protection of semen quality and potency of spermatozoa. Rapa may be more effective than 3-MA in the epididymis against Cis toxicity.
Histopathological Changes In Lung Tissue Caused By Diabetes: A Review
(DergiPark, 2023) Burçin Yıldırım, Ayşegül; Karabulut, Derya; Kaymak, Emin; Kuloğlu, Nurhan; Akin, Ali Tuğrul; Ceylan, Tayfun; Öztürk, Emel
Diabetes mellitus associated with oxidative stress and inflammation can affect many organs. While the effects of diabetes on many organs are well known and documented, its mechanisms of action on the lung are known far less. Hyperglycemia can lead to lung damage by increasing oxidative stresses and inflammation. Diabetes may be a trigger for pulmonary fibrosis, as studies suggest that there may be an important link between pulmonary fibrosis and diabetes. In this review, the histopathological changes caused by diabetes in the lung tissue were summarized. In addition, changes in the lung due to inflammation, oxidative stress and pulmonary fibrosis mechanisms were evaluated.
Investigation of the Effect of Chloroquine on Adriamcycin-Induced Kidney Damage
(2022) Kaymak, Emin; Akin, Ali Tuğrul; Ceylan, Tayfun; Karabulut, Derya
Although Adriamycin (ADR) is an important anticancer drug used in chemotherapy, it causes nephrotoxicity. The inflammation pathway has an important role in ADR-induced nephrotoxicity. Chloroquine (CLQ), which is used as an antimalarial drug, is used in many diseases. Also, CLQ is known as an anti-inflammatory. In this study, we aimed to investigate the effect of CLQ against nephrotoxicity caused by ADR through the inflammatory pathway. Groups were formed as follows; Control (n = 8), CLQ (n = 8) 50 mg / kg intraperitoneally (i.p.) per day, ADR (n = 8) 2 mg / kg i.p. every 3 days, ADR + CLQ (n = 8) 2mg / kg / i.p. ADR + 50 mg / kg / i.p. CLQ. The experiment took a total of 30 days. At the end of the experiment, kidney tissues were taken from the rats under anesthesia. After fixation in the removed kidney tissues, the tissues were embedded in paraffin by histological methods. Sections were taken from kidney tissues. Renal tissue histopathology and Tumor necrosis factor-alpha (TNF-?) and Nuclear factor-?B p65 (NF-?B p65) immunoreactivities were evaluated. When the kidney tissue was examined, it was seen that damage was caused by ADR. In addition, it was observed that TNF-? and NF-?B p65 immunoreactivities in the kidney significantly increased in the ADR group (p<0.05). Damage and inflammatory markers were found to be decreased in the ADR + CLQ group (p <0.05). Chemotherapeutically administered ADR appears to cause nephrotoxicity. CLQ administered was found to reduce this toxicity. As a result, we showed that the damage caused by ADR-induced nephrotoxicity decreased with the application of CLQ through the TNF-? and NF-?B p65 inflammation pathway.
Investigation of the effect of thymoquinone on kidney damage in isoproterenol-induced myocardial infarction in rats and cardiorenal interactions
(Experimental and Applied Medical Science, 2023) Yıldırım, Ayşegül Burçin; Değer, Necla; Sayan, Meryem; Akin, Ali Tuğrul; Ceylan, Tayfun; Kaymak, Emin; Kara, Mehmet; Ünsal, Murat; Karabulut, Derya
This study aimed to determine whether thymoquinone has any protective effects on renal tissue after an isoproterenol-induced myocardial infarction (MI). Experimental groups were formed as 4 groups (n=8). Control group (C). Thymoquinone group (THQ), 20 mg/kg single dose intragastric (i.g.) daily for seven days. Isoproterenol group (ISO) was administered 100 mg/kg intraperitoneally in two doses on days 7 and 8 of the experiment. Thymoquinone+Isoproterenol group (THQ+ISO), THQ 20 mg/kg i.g. was administered once a day for seven days. In addition, two doses of ISO 100 mg/kg i.p. were administered on the seventh and eighth days. Kidney tissues were evaluated histopathologically. Kidney tissues were evaluated histopathologically. Tumour necrosis factor alpha(TNF-?) and alpha Smooth Muscle Actin(?-SMA) immunoreactivity density changes were determined by immunohistochemistry. Glutathione(GST), Glutathione S-transferases(GSTs) and Interleukin-6(IL-6) levels were evaluated by ELISA method. Isoproterenol injection caused severe histopathological changes on kidney tissue. Also TNF-? and ?-SMA levels were found to be higher in groups where ISO was administered. THQ could be effective on kidney tissue to partially correct these histopathological damages, by decreasing fibrosis and inflammation. This study shows that treatment with THQ is effective in preventing kidney damage caused by ISO-induced MI. We think that THQ as a supplementary food will be effective to prevent kidney damage.
Investigation of the Protective Effects of Caffeic Acid Phenethyl Ester against Cisplatin-induced Liver Damage in Rats
(2021) Ceylan, Tayfun; Yakan, Birkan
Cisplatin (CP) is used as an effective chemotherapeutic drug in the treatment of various solid tumors. However, side effects such as hepatotoxicity limit the use of the drug. We investigated the protective effects of caffeic acid phenethyl ester (CAPE), one of the active ingredients of propolis, against hepatotoxicity caused by CP treatment in the liver. 38 Wistar albino rats were divided into 4 groups. Control group was given physiological saline solution for 12 day. CP group was given a single dose of CP (7 mg/kg) on the day 7. CP+CAPE group, was given CAPE (10 ?mol/kg/day) for 12 days and a single dose of CP (7 mg/kg) on day 7. CAPE group received CAPE (10 ?mol/kg/day) for 12 days. Livers of rats sacrificed on the 14th day were stained with hematoxylin-eosin after histological tissue follow-up. The preparations were evaluated and scored. Rat weights were measured and recorded at the beginning and end of the experiment. CP caused significant histopathological changes in the liver. CP also prevented the increase in rat weight. CAPE played an effective role as a protective agent against the histopathological changes caused by CP and showed signs of tissue healing. Our results show that CAPE can be protective against hepatotoxicity associated with CP.
Investigation of the Therapeutic Effects of Chloroquine in Adriamycin-Induced Hepatotoxicity
(Sciendo, 2021) Akin, Ali Tuğrul; Kaymak, Emin; Öztürk, Emel; Karabulut, Derya; Kuloğlu, Nurhan; Ceylan, Tayfun; Toluk, Ayşe
The aim of this study is to investigate the therapeutic effects of Chloroquine (CLQ) against Adriamycin (ADR) induced hepatotoxicity. ADR is a chemotherapeutic agent used in the treatment of many cancer types, but it causes hepatotoxicity. CLQ is used as an anti-inflammatory drug in the treatment of malaria, rheumatoid arthritis, and pneumonia caused by Covid-19. Rats were divided into four groups: Control group, ADR group (2 mg/kg Adriamycin, one in three days for 30 days, i.p.), CLQ group (50 mg/kg Chloroquine, per day for 30 days, i.p.), ADR+CLQ (2 mg/kg Adriamycin, one in three days for 30 days, i.p. and 50 mg/kg Chloroquine, per day for 30 days, i.p.). Animals were sacrificed, and liver tissues were extracted for further examinations. Histopathological changes in liver tissues were scored and IL-17 immunostaining was performed to determine the expression levels among experimental groups. Bodyweights in the ADR group decreased significantly compared to the Control group and CLQ group. Furthermore, bodyweight in ADR+CLQ group was significantly higher compared to ADR group. The histopathological score was significantly higher in ADR group when compared to Control and CLQ group while CLQ administrations reduced the damage induced by ADR in the ADR+CLQ group. IL-17 immunoreactivity was considerably increased in the ADR group. On the other hand, IL-17 expressions of ADR+CLQ were substantially less compared to ADR group. We suggest that CLQ can be used as a therapeutic agent to reduce the detrimental effects of ADR, thanks to its anti-inflammatory properties.

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