EFFECT OF BEE BREAD ON LIVER 5HT2B-MEDIATED GLUCOSE REGULATION IN ALZHEIMER'S RAT MODEL

Abstract

OBJECTIVE: This study aimed to examine the effect of bee bread on glucose regulation and weight change through the change of insulin, serotonin (5-hydroxytryptamine, 5-HT), and leptin hormones in the rat model of Alzheimer's disease (AD).MATERIAL AND METHODS: Alzheimer's disease rat model created via intracerebroventricular (i.c.v.) Streptozotocin (STZ) injection into the lateral ventricles. Beebread administration was performed with daily gavage for three weeks after the STZ injection. Leptin, İnsulin, 5-HT levels in plasma and leptin, insulin, 5-HT, 5HT receptor 2B (5HT2B), glucose transporter 2 (GLUT2), glucose 6-phosphatase (G6pase) levels in liver tissue were measured with Elisa kit. Fasting blood glucose levels were measured using a glucometer, and Homeostatic Model Assessment for Insulin Resistance (HOMA-IR) levels were calculated using the formula. Each rat's weight change was calculated by subtracting their initial weight from their final weight.RESULTS: In the AD-created rats, ıt was observed that blood glucose, plasma insulin, and HOMA-IR levels, liver 5-HT, plasma 5-HT, and leptin levels decreased, liver 5-HT2B and GLUT-2, and weight loss increased. In the AD-created rats, bee bread treatment significantly increased liver 5-HT2B, liver G6pase levels, and plasma leptin levels, also markedly increased plasma 5-HT, liver 5-HT, GLUT-2, and weight loss levels, and decreased fasting blood glucose levels without affecting plasma insülin levels in the AD group.CONCLUSIONS: These results showed that glucose metabolism was modulated to generate an anti-diabetic defense system in the liver tissue of AD-created rats. Beebread administration reduced fasting blood glucose levels by increasing leptin-mediated insulin sensitivity in the AD-created rats.